Short-Cut Method to Assess a Gross Available Energy in a Medium-Load Screw Friction Press

The present study proposed a rapid method, based on a previous universal compression tests, to estimate the required load capacity to cold forge different specimen quantity in a screw press. Accordingly, experimental and theoretical approach are performed to check new adjustable drive motor of the modified forging machine to achieve a gross available energy to deform the specimens preventing damage of the forging machine. During the forging experiments, two screw friction presses (as-received and modified) are used to validate the theoretical approach. The modified press exhibits an increase of 51% of gross energy and 11% of maximum load capacity compare to the as-received press. This method is used to improve the effective of the forging process avoiding excessive loads that could promote machine failure. Therefore, a low-cost and easy to implement methodology is proposed to determine the energy and load capacity of a screw friction press to forge different specimen quantities with symmetry pattern configurations.This work is supported by the Ministry of Economy and Competitiveness of Spain (reference project: FJCI-2016-29297), Instituto Nacional de Tecnologia Industrial (INTI) of Argentina and the Aeronautics Advanced Manufacturing Center (CFAA) of Bilbao

Humans Share More Preferences for Floral Phenotypes With Pollinators Than With Pests.

Studies on the selection of floral traits usually consider pollinators and sometimes herbivores. However, humans also exert selection on floral traits of ornamental plants. We compared the preferences of bumblebees (Bombus terrestris), thrips (Frankliniella occidentalis), and humans for flowers of snapdragon. From a cross of two species, Antirrhinum majus and Antirrhinum linkianum, we selected four Recombinant Inbred Lines (RILs). We characterised scent emission from whole flowers and stamens, pollen content and viability, trichome density, floral shape, size and colour of floral parts. We tested the preferences of bumblebees, thrips, and humans for whole flowers, floral scent bouquets, stamen scent, and individual scent compounds. Humans and bumblebees showed preferences for parental species, whereas thrips preferred RILs. Colour and floral scent, in combination with other floral traits, seem relevant phenotypes for all organisms. Remarkably, visual traits override scent cues for bumblebees, although, scent is an important trait when bumblebees cannot see the flowers, and methyl benzoate was identified as a key attractant for them. The evolutionary trajectory of flowers is the result of multiple floral traits interacting with different organisms with different habits and modes of interaction

Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs

Nowadays, we are facing a renaissance of mitochondria in cancer biology. However, our knowledge of the basic cell biology and on the timing and mechanisms that control the biosynthesis of mitochondrial constituents during progression through the cell cycle of mammalian cells remain largely unknown. Herein, we document the in vivo changes on mitochondrial morphology and dynamics that accompany cellular mitosis, and illustrate the following key points of the biogenesis of mitochondria during progression of liver cells through the cycle: (i) the replication of nuclear and mitochondrial genomes is synchronized during cellular proliferation, (ii) the accretion of OXPHOS proteins is asynchronously regulated during proliferation being the synthesis of β-F1-ATPase and Hsp60 carried out also at G2/M and, (iii) the biosynthesis of cardiolipin is achieved during the S phase, although full development of the mitochondrial membrane potential (ΔΨm) is attained at G2/M. Furthermore, we demonstrate using reporter constructs that the mechanism regulating the accretion of β-F1-ATPase during cellular proliferation is controlled at the level of mRNA translation by the 3′UTR of the transcript. The 3′UTR-driven synthesis of the protein at G2/M is essential for conferring to the daughter cells the original phenotype of the parental cell. Our findings suggest that alterations on this process may promote deregulated β-F1-ATPase expression in human cancer

Epidemiological trends of HIV/HCV coinfection in Spain, 2015-2019

Altres ajuts: Spanish AIDS Research Network; European Funding for Regional Development (FEDER).Objectives: We assessed the prevalence of anti-hepatitis C virus (HCV) antibodies and active HCV infection (HCV-RNA-positive) in people living with HIV (PLWH) in Spain in 2019 and compared the results with those of four similar studies performed during 2015-2018. Methods: The study was performed in 41 centres. Sample size was estimated for an accuracy of 1%. Patients were selected by random sampling with proportional allocation. Results: The reference population comprised 41 973 PLWH, and the sample size was 1325. HCV serostatus was known in 1316 PLWH (99.3%), of whom 376 (28.6%) were HCV antibody (Ab)-positive (78.7% were prior injection drug users); 29 were HCV-RNA-positive (2.2%). Of the 29 HCV-RNA-positive PLWH, infection was chronic in 24, it was acute/recent in one, and it was of unknown duration in four. Cirrhosis was present in 71 (5.4%) PLWH overall, three (10.3%) HCV-RNA-positive patients and 68 (23.4%) of those who cleared HCV after anti-HCV therapy (p = 0.04). The prevalence of anti-HCV antibodies decreased steadily from 37.7% in 2015 to 28.6% in 2019 (p < 0.001); the prevalence of active HCV infection decreased from 22.1% in 2015 to 2.2% in 2019 (p < 0.001). Uptake of anti-HCV treatment increased from 53.9% in 2015 to 95.0% in 2019 (p < 0.001). Conclusions: In Spain, the prevalence of active HCV infection among PLWH at the end of 2019 was 2.2%, i.e. 90.0% lower than in 2015. Increased exposure to DAAs was probably the main reason for this sharp reduction. Despite the high coverage of treatment with direct-acting antiviral agents, HCV-related cirrhosis remains significant in this population

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe

Differential effects of nutrient enrichment on carbon metabolism and dissolved organic carbon (DOC) fluxes in macrophytic benthic communities

Nutrient enrichment can alter negatively benthic communities and reduce their ecological services. This study explores in two contrasting seasons (winter and summer), the effects of in situ nutrient enrichment at the community level on carbon metabolism and dissolved organic carbon (DOC) fluxes in two benthic communities dominated by the seagrass Cymodocea nodosa and by the macroalga Caulerpa prolifera. Under nutrient enrichment, C. nodosa increased total community biomass and diversity in summer, while net community production shifted from net autotrophic to net heterotrophic in winter. In contrast, C. prolifera became heterotrophic in summer, while no significant effects were found in winter. Regarding DOC fluxes, nutrient enrichment shifted C. nodosa from net DOC consumer in winter to a strong net DOC producer in summer, while C. prolifera seemed unaffected. Therefore, nutrient enrichment can promote conditional effects (positive, negative or neutral) in coastal areas depending both on macrophyte assemblages and season.This study was funded by the national projects CTM 2011-24482 (SEA-LIVE) and CTM 2017-85365-R (PAVAROTTI) and by the Excellence Project of Junta Andalucia RNM-P12-3020 (PRODESCA)

Leaf Senescence of the Seagrass Cymodocea nodosa in C&aacute;diz Bay, Southern Spain

Leaf decay in seagrasses is enhanced in some seasons since large green senescent beach-cast seagrass leaves are frequently recorded during autumn and winter seasons. Here, we explore if senescence is operating in seagrass leaf decay or if hydrodynamic stress is responsible for the seasonal leaf abscission. A seasonal study on the temperate seagrass Cymodocea nodosa was carried out in four locations with contrasting hydrodynamic regimes. The morphological, biomechanical and material properties of C. nodosa were measured. The force required to break the ligule was always lower than that required to break the blade. This could be considered an adaptive strategy to reduce acute drag forces and thus lessen the chance of plant uprooting. The absolute force needed to dislodge the blade at the ligule level varied with season and location, with the lowest forces recorded in autumn. This may indicate that senescence is operating in this species. On the other hand, the minimum estimated failure velocities for leaf abscission were also recorded in autumn. Consequently, this may cause the premature shedding of leaves in this season before the senescence process has finished and can probably explain the occurrence of green beach-cast seagrass leaves usually found during autumn and winter

Leaf Senescence of the Seagrass <i>Cymodocea nodosa</i> in Cádiz Bay, Southern Spain

Leaf decay in seagrasses is enhanced in some seasons since large green senescent beach-cast seagrass leaves are frequently recorded during autumn and winter seasons. Here, we explore if senescence is operating in seagrass leaf decay or if hydrodynamic stress is responsible for the seasonal leaf abscission. A seasonal study on the temperate seagrass Cymodocea nodosa was carried out in four locations with contrasting hydrodynamic regimes. The morphological, biomechanical and material properties of C. nodosa were measured. The force required to break the ligule was always lower than that required to break the blade. This could be considered an adaptive strategy to reduce acute drag forces and thus lessen the chance of plant uprooting. The absolute force needed to dislodge the blade at the ligule level varied with season and location, with the lowest forces recorded in autumn. This may indicate that senescence is operating in this species. On the other hand, the minimum estimated failure velocities for leaf abscission were also recorded in autumn. Consequently, this may cause the premature shedding of leaves in this season before the senescence process has finished and can probably explain the occurrence of green beach-cast seagrass leaves usually found during autumn and winter

Effects of ocean acidification and hydrodynamic conditions on carbon metabolism and dissolved organic carbon (DOC) fluxes in seagrass populations - Fig 2

<p><b>Effects of seawater acidification and current velocity on (A) gross primary production (GPP), (B) respiration (R) and (C) net primary production (NPP).</b> LV = Low velocity, MV = Medium velocity, HV = High velocity, CpH = Current pH, FpH = Forecasted pH.</p